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SOX9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24659

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Goat, Primate, Sheep

Cited:

Human, Mouse, Ovine

Predicted:

Bovine (93%), Chimpanzee (93%), Equine (100%), Monkey (100%), Opossum (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A portion of amino acids 225-275 of human SOX9 was used as the immunogen for this antibody.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29624608). The amino acid sequence used as immunogen for the ZBTB2 antibody is 100% homologous in monkey, opossum and horse, and 93% homologous in cow and chimpanzee.

Marker

Sertoli Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX9 Antibody

Simple Western: SOX9 Antibody [NBP2-24659]

Simple Western: SOX9 Antibody [NBP2-24659]

Simple Western: SOX9 Antibody [NBP2-24659] - Simple Western lane view shows a specific band for SOX9 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659] - Osteo-chondroprogenitor cells in the zone adjacent to the titanium plate surface are positive for Sox9 (arrows). Picture on the left represent Group 0.1, pictures on the right represent Group 0.6. 100-fold (c-d) magnification. PLoS One. 2018 Apr 6;13(4):e0195466. doi: 10.1371/journal.pone.0195466.
Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659] - 3'-Sialyllactose regulates anabolic and catabolic factor expression via Erk and NF-kB pathways. (C) Immunohistochemical staining of Sox9 in DMM-induced OA mice with administration of PBS and 3'-sialyllactose. Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1111/jcmm.13292), licensed under a CC-BY license.

Applications for SOX9 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reviewed Applications

Read 1 review rated 3 using NBP2-24659 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

UniProt

Additional SOX9 Products

Product Documents for SOX9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOX9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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