SP-B/Surfactant Protein B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11269

Novus Biologicals, part of Bio-Techne
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NBP3-11269
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.1 mg/ml

View all SP-B/Surfactant Protein B Antibodies »

Product Specifications

Immunogen

The polyclonal antibody is raised against porcine SP-B from porcine lungs which has been reconstituted in micelles of lysophosphatidylcholine.

Specificity

The antibody rescognizes under nonreducing conditions the most prominent forms of SP-B of 8 kD and 22 kD, corresponding to SP-B dimers.

Clonality

Polyclonal

Host

Rabbit

Applications for SP-B/Surfactant Protein B Antibody

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SP-B/Surfactant Protein B

The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behaviour in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). The polyclonal antibody is raised against pig SP-B from pig lungs which has been reconstituted in micelles of lysophosphatidylcholine. The antibody rescognizes under nonreducing conditions the most prominent forms of SP-B of 8 kD and 22 kD, corresponding to SP-B dimers. The polyclonal antibody is cross reactive with human SP-B.

Long Name

Surfactant Protein B

Alternate Names

PSP-B, SFTB3, SFTP3, SFTPB, SMDP1

Gene Symbol

SFTPB

Additional SP-B/Surfactant Protein B Products

Product Documents for SP-B/Surfactant Protein B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SP-B/Surfactant Protein B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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