SP110 Antibody (PSH03-66)

Western Blot: SP110 Antibody (PSH03-66) [NBP3-32973]

Western Blot: SP110 Antibody (PSH03-66) [NBP3-32973] - Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (NBP3-32973) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate
Lane 3: HL-60 cell lysate
Lane 4: SW480 cell lysate
Lane 5: Daudi cell lysate
Lane 6: K-562 cell lysate
Lane 7: PC-3M cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 78 kDa
Observed band size: 29-78 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32973) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SP110 Antibody (PSH03-66) [NBP3-32973]

Immunohistochemistry: SP110 Antibody (PSH03-66) [NBP3-32973] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (NBP3-32973) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32973) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.