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SP140 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49517

Novus Biologicals, part of Bio-Techne
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NBP2-49517
NBP2-49517-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELENHPMNEEGESEELASSLLYDNVPGAEQSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP140 Antibody

Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517]

Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517]

Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517] - Staining in human spleen and cerebral cortex tissues using anti-SP140 antibody. Corresponding SP140 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: SP140 Antibody [NBP2-49517]

Immunocytochemistry/ Immunofluorescence: SP140 Antibody [NBP2-49517]

Immunocytochemistry/Immunofluorescence: SP140 Antibody [NBP2-49517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.
Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517]

Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517]

Immunohistochemistry-Paraffin: SP140 Antibody [NBP2-49517] - Staining of human cerebral cortex shows low expression as expected.

Applications for SP140 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP140

SP140 is a component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection

Alternate Names

Lymphoid-restricted homolog of Sp100, lymphoid-specific SP100 homolog, LYSP100, LYSP100-A, LYSP100-B, MGC126440, Nuclear autoantigen Sp-140, nuclear body protein SP140, SP140 nuclear body protein, Speckled 140 kDa

Gene Symbol

SP140

Additional SP140 Products

Product Documents for SP140 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SP140 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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