SP17 Antibody (3G9F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27160

Novus Biologicals, part of Bio-Techne
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NBP3-27160-0.025ml
NBP3-27160-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G9F3

Concentration

1 mg/ml

View all SP17 Antibodies »

Product Summary for SP17 Antibody (3G9F3)

Immunogen

Purified recombinant fragment of human SP17 (AA: 1-152) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SP17 Antibody (3G9F3)

Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] -

Flow Cytometry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of SK-OV-3 cells using SP17 mouse mAb (green) and negative control (red).

Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] -

Western Blot: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis using SP17 mAb against human SP17 (AA: 1-152) recombinant protein. (Expected MW is 20.3 kDa)

Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] -

Immunohistochemistry: SP17 Antibody (3G9F3) [NBP3-27160] - Analysis of paraffin-embedded rectal cancer tissues using SP17 mouse mAb with DAB staining.
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Applications for SP17 Antibody (3G9F3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP17

SP17 encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Alternate Names

Cancer/testis antigen 22, CT22SP17-1, Sp17-1, SP17sperm surface protein Sp17, sperm autoantigenic protein 17Sperm protein 17

Gene Symbol

SPA17

Additional SP17 Products

Product Documents for SP17 Antibody (3G9F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SP17 Antibody (3G9F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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