SP17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00053340-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP17 Antibodies »

Product Specifications

Immunogen

SPA17 (NP_059121.1, 1 a.a. - 151 a.a.) full-length human protein. MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAKKMKTNSLQNEEKEENK

Specificity

Reacts with sperm autoantigenic protein 17.

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for SP17 Antibody

Western Blot: SP17 Antibody [H00053340-B01P]

Western Blot: SP17 Antibody [H00053340-B01P]

Western Blot: SP17 Antibody [H00053340-B01P] - Analysis of SPA17 expression in transfected 293T cell line by SPA17 polyclonal antibody. Lane 1: SPA17 transfected lysate(16.61 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SP17 Antibody [H00053340-B01P]

Immunocytochemistry/ Immunofluorescence: SP17 Antibody [H00053340-B01P]

Immunocytochemistry/Immunofluorescence: SP17 Antibody [H00053340-B01P] - Analysis of purified antibody to SPA17 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: SP17 Antibody [H00053340-B01P]

Immunohistochemistry-Paraffin: SP17 Antibody [H00053340-B01P]

Immunohistochemistry-Paraffin: SP17 Antibody [H00053340-B01P] - Analysis of purified antibody to SPA17 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)

Applications for SP17 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
This antibody is reactive against transfected lysate in western blot, immunofluorescence, immunohistochemistry (paraffin sections), and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP17

This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Alternate Names

Cancer/testis antigen 22, CT22SP17-1, Sp17-1, SP17sperm surface protein Sp17, sperm autoantigenic protein 17Sperm protein 17

Entrez Gene IDs

53340 (Human)

Gene Symbol

SPA17

UniProt

NP_059121.1 (Human)

Additional SP17 Products

Product Documents for SP17 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SP17 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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