SP2 Antibody (5D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00006668-M01-100ug

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP2 Antibodies »

Product Specifications

Immunogen

SP2 (NP_003101.2, 71 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPGKNSFGILSSKGNILQIQGSQLSASYPGGQLVFAIQNPTMINKGTRSNANIQYQAVPQIQASNSQTIQVQPNLTNQIQIIPGTNQAIIT

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SP2 Antibody (5D3)

Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug]

Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug]

Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug] - Detection against Immunogen (35.75 KDa) .
Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug]

Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug]

Western Blot: SP2 Antibody (5D3) [H00006668-M01-100ug] - Analysis of SP2 expression in transfected 293T cell line by SP2 monoclonal antibody (M01), clone 5D3. Lane 1: SP2 transfected lysate (Predicted MW: 25.6 KDa). Lane 2: Non-transfected lysate.
ELISA: SP2 Antibody (5D3) [H00006668-M01-100ug]

ELISA: SP2 Antibody (5D3) [H00006668-M01-100ug]

ELISA: SP2 Antibody (5D3) [H00006668-M01-100ug] - Detection limit for recombinant GST tagged SP2 is 0.03 ng/ml as a capture antibody.

Applications for SP2 Antibody (5D3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP2

The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3.

Alternate Names

KIAA0048, Sp2 transcription factor, transcription factor Sp2

Entrez Gene IDs

6668 (Human)

Gene Symbol

SP2

OMIM

601801 (Human)

UniProt

NP_003101.2 (Human)

Additional SP2 Products

Product Documents for SP2 Antibody (5D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SP2 Antibody (5D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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