SPAG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38707

Novus Biologicals, part of Bio-Techne
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NBP2-38707
NBP2-38707-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPAG1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KKTPRDYAEWDKFDVEKECLKIDEDYKEKTVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAG1 Antibody

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707] - Staining in human testis and prostate tissues using anti-SPAG1 antibody. Corresponding SPAG1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707]

Immunohistochemistry-Paraffin: SPAG1 Antibody [NBP2-38707] - Staining of human prostate shows low expression as expected.

Applications for SPAG1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAG1

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

Alternate Names

FLJ32920, HSD-3.8sperm-associated antigen 1, Infertility-related sperm protein Spag-1, SP75, sperm associated antigen 1, tetratricopeptide repeat-containing protein, TPIS, TPR-containing protein involved in spermatogenesis

Gene Symbol

SPAG1

UniProt

Q07617 (Human)

Additional SPAG1 Products

Product Documents for SPAG1 Antibody

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Certificate of Analysis

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Product Specific Notices for SPAG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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