SPAG16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84124

Novus Biologicals, part of Bio-Techne
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NBP1-84124
NBP1-84124-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPAG16 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLKKDLKHYKQAADKAREDLLKIQKERDFHRMHHKRIVQEKNKLINDLKGLKLHYASYEPTIRVLHEKHHTLLKEKMLTSLERDKVVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAG16 Antibody

Western Blot: SPAG16 Antibody [NBP1-84124]

Western Blot: SPAG16 Antibody [NBP1-84124]

Western Blot: SPAG16 Antibody [NBP1-84124] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: SPAG16 Antibody [NBP1-84124]

Immunocytochemistry/ Immunofluorescence: SPAG16 Antibody [NBP1-84124]

Immunocytochemistry/Immunofluorescence: SPAG16 Antibody [NBP1-84124] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: SPAG16 Antibody [NBP1-84124]

Immunohistochemistry-Paraffin: SPAG16 Antibody [NBP1-84124]

Immunohistochemistry-Paraffin: SPAG16 Antibody [NBP1-84124] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.

Applications for SPAG16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAG16

Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM]

Alternate Names

DKFZp666P1710, FLJ22724, FLJ37717, Pf20 protein homolog, PF20MGC87036, sperm associated antigen 16, sperm-associated antigen 16 protein, sperm-associated WD repeat protein, WD repeat domain 29, WDR29

Gene Symbol

SPAG16

Additional SPAG16 Products

Product Documents for SPAG16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPAG16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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