SPAG16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97844

Novus Biologicals, part of Bio-Techne
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NBP2-97844-100ul
NBP2-97844-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPAG16 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human SPAG16 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SPAG16 Antibody

Western Blot: SPAG16 Antibody [NBP2-97844]

Western Blot: SPAG16 Antibody [NBP2-97844]

Western Blot: SPAG16 Antibody [NBP2-97844] - Anti-SPAG16 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence: SPAG16 Antibody [NBP2-97844]

Immunocytochemistry/ Immunofluorescence: SPAG16 Antibody [NBP2-97844]

Immunocytochemistry/Immunofluorescence: SPAG16 Antibody [NBP2-97844] - Immunofluorescence staining of SPAG16 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SPAG16 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for SPAG16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAG16

Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM]

Alternate Names

DKFZp666P1710, FLJ22724, FLJ37717, Pf20 protein homolog, PF20MGC87036, sperm associated antigen 16, sperm-associated antigen 16 protein, sperm-associated WD repeat protein, WD repeat domain 29, WDR29

Gene Symbol

SPAG16

Additional SPAG16 Products

Product Documents for SPAG16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPAG16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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