SPAG6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80923

Novus Biologicals, part of Bio-Techne
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NBP1-80923
NBP1-80923-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPAG6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEA

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:30865884).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAG6 Antibody

Western Blot: SPAG6 Antibody [NBP1-80923]

Western Blot: SPAG6 Antibody [NBP1-80923]

Western Blot: SPAG6 Antibody [NBP1-80923] - Analysis in control (vector only transfected HEK293T lysate) and SPAG6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923]

Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923]

Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923] - Staining in human fallopian tube and colon tissues using anti-SPAG6 antibody. Corresponding SPAG6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923]

Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923]

Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP1-80923] - Staining of human colon shows low expression as expected.
View 1 more images

Applications for SPAG6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Western Blot verified by a customer review.

Reviewed Applications

Read 1 review rated 3 using NBP1-80923 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPAG6

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Alternate Names

axoneme central apparatus protein, DKFZp434I153, pf16, Protein PF16 homolog, Repro-SA-1MGC26276, sperm associated antigen 6, Sperm flagellar protein, sperm-associated antigen 6

Entrez Gene IDs

9576 (Human)

Gene Symbol

SPAG6

UniProt

O75602 (Human)

Additional SPAG6 Products

Product Documents for SPAG6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SPAG6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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