SPAG8 Antibody (2F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00026206-M01

Novus Biologicals, part of Bio-Techne
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H00026206-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPAG8 Antibodies »

Product Specifications

Immunogen

SPAG8 (AAH19247, 1 a.a. ~ 501 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. METNGSTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSALAAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGFTHNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVLNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLPRGQCLLYNWEEERATNHLDQVPSMQDGSESFFFRHGHRGLLTMQLKSPMPSSTTQKDSYQPPGNVYWPLRGKREAMLEMLLQHQICKEVQAEQEPTRKLFEVESVTHHDYRMELAQAGTPAPTKPHDYRQEQPETFWIQRAPQLPTWWPLPTQVPAAEDYLTWKEWGFTGVQEVLSALLRATPGEYSVNICGMNEHPVCSRTWTNRLCHQEMGSKKTVTQEDRGW

Specificity

SPAG8 - sperm associated antigen 8 (2F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SPAG8 Antibody (2F12)

Western Blot: SPAG8 Antibody (2F12) [H00026206-M01]

Western Blot: SPAG8 Antibody (2F12) [H00026206-M01]

Western Blot: SPAG8 Antibody (2F12) [H00026206-M01] - Analysis of SPAG8 expression in transfected 293T cell line by SPAG8 monoclonal antibody (M01), clone 2F12.Lane 1: SPAG8 transfected lysate(53.5 KDa).Lane 2: Non-transfected lysate.

Applications for SPAG8 Antibody (2F12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPAG8

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq]

Alternate Names

BS-84, HSD-1MGC26201, hSMP-1, SMP1, SMP-1, SPAG3, sperm associated antigen 8, Sperm membrane protein 1, Sperm membrane protein BS-84, sperm-associated antigen 8

Entrez Gene IDs

26206 (Human)

Gene Symbol

SPAG8

UniProt

AAH19247 (Human)

Additional SPAG8 Products

Product Documents for SPAG8 Antibody (2F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPAG8 Antibody (2F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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