SPANXC Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77925
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Protein A purified SPANXC Antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANXC protein. (Uniprot: Q9NY87)
Reactivity Notes
A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANXC proteins from human, gorilla and chimpanzee. Reactivity against homologues from is not known.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This Protein A purified antibody is directed against human SPANXC protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SPANXC Antibody
![Western Blot: SPANXC Antibody [NBP1-77925]](https://resources.bio-techne.com/images/products/SPANXC-Antibody-Western-Blot-NBP1-77925-img0002.jpg "Western Blot: SPANXC Antibody [NBP1-77925]")
Western Blot: SPANXC Antibody [NBP1-77925]
Western Blot: SPANXC Antibody [NBP1-77925] - Shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.![Immunohistochemistry: SPANXC Antibody [NBP1-77925]](https://resources.bio-techne.com/images/products/SPANXC-Antibody-Immunohistochemistry-NBP1-77925-img0004.jpg "Immunohistochemistry: SPANXC Antibody [NBP1-77925]")
Immunohistochemistry: SPANXC Antibody [NBP1-77925]
Immunohistochemistry: SPANXC Antibody [NBP1-77925] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
SPANXC Antibody
Western blot using Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX () was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.Applications for SPANXC Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunohistochemistry
1.25-2.5 ug/ml
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue. Most human cell lines are SPANX negative.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPANXC
Alternate Names
B1, C, cancer/testis antigen 11.3, cancer/testis antigen family 11, member 3, cancer/testis-associated protein CTp11, cancer/testis-associated protein of 11 kD, cancer-testis-associated protein CTp11, CT11.1, CT11.2, CT11.3, CT11.4, CTp11, dJ171K16.1, NAP-X, nuclear-associated protein SPAN-Xc, SPANX, SPANX family, member C, SPANXA, SPAN-Xa, SPANX-A, SPANXB, SPAN-Xb, SPANX-B, SPANX-C, SPAN-Xc protein, SPANX-D, sperm protein associated with the nucleus on the X chromosome C, sperm protein associated with the nucleus, X chromosome, family member C
Gene Symbol
SPANXC
UniProt
Additional SPANXC Products
Product Documents for SPANXC Antibody
Product Specific Notices for SPANXC Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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