SPARC Antibody (rOSTN/8527)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20672
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rOSTN/8527
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
Localization
Secreted. Basement membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide store at 2 to 8C. Antibody without azide store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for SPARC Antibody (rOSTN/8527)
SPARC Antibody (rOSTN/8527)
Formalin-fixed, paraffin-embedded human spleen stained with SPARC antibody (rOSTN/8527). Inset: PBS instead of primary antibody; secondary only negative control.Applications for SPARC Antibody (rOSTN/8527)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SPARC
Long Name
Secreted Protein Acidic and Rich in Cysteine
Alternate Names
BM-40, Osteonectin
Gene Symbol
SPARC
Additional SPARC Products
Product Documents for SPARC Antibody (rOSTN/8527)
Product Specific Notices for SPARC Antibody (rOSTN/8527)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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