SPARC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27358

Novus Biologicals, part of Bio-Techne
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NBP2-27358
NBP2-27358SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Orangutan (97%), Porcine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SPARC Antibodies »

Product Specifications

Immunogen

A portion of amino acids 25-85 of human SPARC was used as the immunogen for the antibody. (aa46-63).

Reactivity Notes

The amino acid sequence used as immunogen is 86% homologous in rabbit.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPARC Antibody

Western Blot: SPARC Antibody [NBP2-27358]

Western Blot: SPARC Antibody [NBP2-27358]

Western Blot: SPARC Antibody [NBP2-27358] - Analysis of human A375 lysate probed with SPARC antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358] - FFPE human placenta tissue stained with SPARC antibody, peroxidase-conjugate and DAB chromogen.

Applications for SPARC Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

2-5ug/ml~
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPARC

SPARC, also called Osteonectin, is an extracellular matrix (ECM) associated glycoprotein. SPARC activates matrix metalloproteinase-2, a key effector of ECM proteolysis, a process linked to enhanced tumor invasion and metastatic potential (1). MMP expression by tumor cells themselves may be representative of more invasive migratory cancers or latter stages of tumor progression (2, 3). SPARC appears to enhance MMP-2 activation at least in invasive breast cancer cell lines. Levels of SPARC expression appear to be directly correlated with progressive cancers not only of breast, but colon, kidney, ovary and brain (4, 5). SPARC specific antibody may establish levels of expression of SPARC providing its' use as a biomarker of late stage or aggressive tumors.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

UniProt

P09486

Additional SPARC Products

Product Documents for SPARC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPARC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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