SPDEF Antibody (4A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00025803-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SPDEF (NP_036523, 92 a.a. ~ 200 a.a) partial recombinant protein with GST tag. QCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMVVGEVLKD IETACKLLNITADPMDWSPSNVQKWLLWTEHQYRLPPMGKAF QELAGKELCAMSEEQFRQRSPLGGD
Specificity
SPDEF - SAM pointed domain containing ets transcription factor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for SPDEF Antibody (4A5)
![Western Blot: SPDEF Antibody (4A5) [H00025803-M01]](https://resources.bio-techne.com/images/products/SPDEF-Antibody-4A5-Western-Blot-H00025803-M01-img0003.jpg "Western Blot: SPDEF Antibody (4A5) [H00025803-M01]")
Western Blot: SPDEF Antibody (4A5) [H00025803-M01]
Western Blot: SPDEF Antibody (4A5) [H00025803-M01] - Analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SPDEF Antibody (4A5) [H00025803-M01] - Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.
Applications for SPDEF Antibody (4A5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SPDEF
Alternate Names
bA375E1.3, PDEFRP11-375E1__A.3, Prostate epithelium-specific Ets transcription factor, Prostate-derived Ets factor, Prostate-specific Ets, PSE, SAM pointed domain containing ets transcription factor, SAM pointed domain-containing Ets transcription factor
Entrez Gene IDs
25803 (Human)
Gene Symbol
SPDEF
OMIM
608144 (Human)
UniProt
Additional SPDEF Products
Product Documents for SPDEF Antibody (4A5)
Product Specific Notices for SPDEF Antibody (4A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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