Spectrin beta 1 Antibody (4C3)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-574

Novus Biologicals, part of Bio-Techne
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NB300-574
NB300-574-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Canine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C3

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Spectrin beta 1 Antibodies »

Product Specifications

Immunogen

Purified human erythrocyte beta-1 spectrin.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24505439). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Detects spectrin from erythrocytes, brain and muscle cells. This has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Spectrin beta 1 Antibody (4C3)

Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574]

Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574]

Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.
Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574]

Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574]

Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574]

Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle. Otherwise normal, Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD) muscle biopsy sections were stained for Neu5Gc (green),Spectrin beta 1 (red) and DAPI (blue). Arrows indicate Neu5Gc puncta in cytoplasmic or perimembranous regions of BMD and DMD skeletal myofibers. Bar is 50 um for all panels. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0088226) licensed under a CC-BY license.
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Applications for Spectrin beta 1 Antibody (4C3)

Application
Recommended Usage

Flow Cytometry

0.5 - 2 ug

Immunocytochemistry/ Immunofluorescence

1:10 - 1:200

Immunohistochemistry

1:20 - 1:200

Immunohistochemistry-Frozen

1:20 - 1:200

Immunohistochemistry-Paraffin

1:20 - 1:200

Western Blot

1:100
Application Notes
WB: Detects an approx. 246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. IF: Staining of beta-1 spectrin in rat skeletal muscle fibers results in intense staining of the sarcolemma. Antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution. IHC usage was reported in scientitic literature (PMID: 9142435). IHC-Fr usage was reported in scientific literature (PMID: 24505439).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Spectrin beta 1

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Alternate Names

beta-I spectrin, EL3, erythrocyte, HS2, HSPTB1, membrane cytoskeletal protein, spectrin, beta, erythrocytic, SPTB1

Entrez Gene IDs

6710 (Human); 314251 (Rat)

Gene Symbol

SPTB

Additional Spectrin beta 1 Products

Product Documents for Spectrin beta 1 Antibody (4C3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin beta 1 Antibody (4C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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