Spectrin beta 1 Antibody (RG/26) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08440CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP3-07292

Alexa Fluor 350

NBP3-08440AF350

Alexa Fluor 405

NBP3-08440AF405

Alexa Fluor 488

NBP3-08440AF488

Alexa Fluor 532

NBP3-08440AF532

Alexa Fluor 594

NBP3-08440AF594

Alexa Fluor 647

NBP3-08440AF647

Alexa Fluor 700

NBP3-08440AF700

Alexa Fluor 750

NBP3-08440AF750

Allophycocyanin

NBP3-08440APC

Biotin

NBP3-08440B

DyLight 350

NBP3-08440UV

DyLight 405

NBP3-08440V

DyLight 488

NBP3-08440G

DyLight 550

NBP3-08440R

DyLight 594

NBP3-08440DL594

DyLight 650

NBP3-08440C

DyLight 680

NBP3-08440FR

DyLight 755

NBP3-08440IR

FITC

NBP3-08440F

HRP

NBP3-08440H

Janelia Fluor 525

NBP3-08440JF525

Janelia Fluor 549

NBP3-08440JF549

Janelia Fluor 585

NBP3-08440JF585

Janelia Fluor 635

NBP3-08440JF635

Janelia Fluor 646

NBP3-08440JF646

Janelia Fluor 669

NBP3-08440JF669

PE

NBP3-08440PE

PerCP

NBP3-08440PCP

Forumulation

Catalog #

Azide and BSA Free

NBP3-08440

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # RG/26

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Spectrin beta 1 Antibodies »

Product Summary for Spectrin beta 1 Antibody (RG/26) [CoraFluor™ 1]

Immunogen

Red cell membranes

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Spectrin beta 1 Antibody (RG/26) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Spectrin beta 1

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Alternate Names

beta-I spectrin, EL3, erythrocyte, HS2, HSPTB1, membrane cytoskeletal protein, spectrin, beta, erythrocytic, SPTB1

Gene Symbol

SPTB

Additional Spectrin beta 1 Products

Product Documents for Spectrin beta 1 Antibody (RG/26) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Spectrin beta 1 Antibody (RG/26) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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