Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59668

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-59668
NBP2-59668SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1287A

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all beta-III Spectrin Antibodies »

Product Summary for Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

Immunogen

Synthetic peptide containing the C-terminal region of human Spectrin beta 3 (UniProt O15020)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

Western Blot: Spectrin beta 3 Antibody (1287A)Azide and BSA Free [NBP2-59668]

Western Blot: Spectrin beta 3 Antibody (1287A)Azide and BSA Free [NBP2-59668]

Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (1287A) - Azide and BSA Free [NBP2-59668]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody (1287A) - Azide and BSA Free [NBP2-59668]

Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (1287A) at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) - Azide and BSA Free [NBP2-59668]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) - Azide and BSA Free [NBP2-59668]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human brain using Spectrin beta III antibody at a 1:1,000 dilution. The primary antibody bound to Spectrin beta III was detected with HRP-DAB detection method and the nuclei were counterstained with hematoxylin. This Spectrin beta III antibody generated a specific cytoplasmic staining in all the cells with strongest signal in neuronal cells.
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Applications for Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

1 ug/mL

Immunocytochemistry/ Immunofluorescence

5 - 20 ug/ml

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-III Spectrin

Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).

Long Name

Spectrin Beta, Non-Erythrocytic 2

Alternate Names

GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2

Gene Symbol

SPTBN2

Additional beta-III Spectrin Products

Product Documents for Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin beta 3 Antibody (1287A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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