Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54488
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582)Azide and BSA Free [NBP2-54488]](https://resources.bio-techne.com/images/products/Spectrin-beta-3-Antibody-SPTBN2-1582-Azide-and-BSA-Free-Western-Blot-NBP2-54488-img0001.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582)Azide and BSA Free [NBP2-54488]")
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]](https://resources.bio-techne.com/images/products/Spectrin-beta-3-Antibody-SPTBN2-1582-Azide-and-BSA-Free-Protein-Array-NBP2-54488-img0004.jpg "Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]")
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582)Azide and BSA Free [NBP2-54488]](https://resources.bio-techne.com/images/products/Spectrin-beta-3-Antibody-SPTBN2-1582-Azide-and-BSA-Free-Western-Blot-NBP2-54488-img0005.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582)Azide and BSA Free [NBP2-54488]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPTBN2/1582
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa356-475) of human Spectrin beta 3 protein (exact sequence is proprietary) (Uniprot: O15020)
Localization
Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
246 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53236).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582)Azide and BSA Free [NBP2-54488]
Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]](https://resources.bio-techne.com/images/products/Spectrin-beta-3-Antibody-SPTBN2-1582-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54488-img0006.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]")
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Antibody (SPTBN2/1582).![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]](https://resources.bio-techne.com/images/products/Spectrin-beta-3-Antibody-SPTBN2-1582-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54488-img0003.jpg "Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]")
Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488]
Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free [NBP2-54488] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
Application
Recommended Usage
ELISA
2 - 4 ug/ml
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: beta-III Spectrin
Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. Mutations in spectrins are a previously unknown cause of ataxia and neurodegenerative disease that affect membrane proteins involved in glutamate signaling. Spectrin-beta IIIs have been recognized as ataxia disease genes and their mutations cause spinocerebellar ataxia type 5 (SCA5).
Long Name
Spectrin Beta, Non-Erythrocytic 2
Alternate Names
GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2
Gene Symbol
SPTBN2
Additional beta-III Spectrin Products
Product Documents for Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
Product Specific Notices for Spectrin beta 3 Antibody (SPTBN2/1582) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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