SPEN Antibody [Janelia Fluor® 585]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-58799JF585

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Proximity Ligation Assay, Western Blot

Label

Janelia Fluor 585

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPEN Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 3375 and 3425 of human SMART/HDAC1 associated repressor protein (spen homolog, transcriptional regulator) using the numbering given in entry NP_055816.2 (GeneID 23013).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29880719).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SPEN Antibody [Janelia Fluor® 585]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SPEN

SPEN encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Alternate Names

HIAA0929, KIAA0929nuclear receptor transcription cofactor, MINTMsx2 interacting nuclear target (MINT) homolog, RBM15C, SHARPmsx2-interacting protein, SMART/HDAC1 associated repressor protein, SMART/HDAC1-associated repressor protein, SPEN homolog, spen homolog, transcriptional regulator (Drosophila)

Gene Symbol

SPEN

Additional SPEN Products

Product Documents for SPEN Antibody [Janelia Fluor® 585]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPEN Antibody [Janelia Fluor® 585]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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