SPEN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82952

Novus Biologicals, part of Bio-Techne
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NBP1-82952
NBP1-82952-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPEN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25915022).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPEN Antibody

Immunocytochemistry/ Immunofluorescence: SPEN Antibody [NBP1-82952]

Immunocytochemistry/ Immunofluorescence: SPEN Antibody [NBP1-82952]

Immunocytochemistry/Immunofluorescence: SPEN Antibody [NBP1-82952] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952]

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952]

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952]

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952]

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
View 2 more images

Applications for SPEN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

Reported in scientific literature (PMID 25915022)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPEN

SPEN encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Alternate Names

HIAA0929, KIAA0929nuclear receptor transcription cofactor, MINTMsx2 interacting nuclear target (MINT) homolog, RBM15C, SHARPmsx2-interacting protein, SMART/HDAC1 associated repressor protein, SMART/HDAC1-associated repressor protein, SPEN homolog, spen homolog, transcriptional regulator (Drosophila)

Gene Symbol

SPEN

Additional SPEN Products

Product Documents for SPEN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPEN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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