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Spermine synthase Antibody (OTI3C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00900

Novus Biologicals, part of Bio-Techne
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NBP2-00900

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3C9

Concentration

0.92 mg/ml

Product Summary for Spermine synthase Antibody (OTI3C9)

Immunogen

Full length human recombinant protein of human SMS(NP_004586) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Spermine synthase Antibody (OTI3C9)

Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Western Blot: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Spermine synthase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Spermine synthase.
Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Immunohistochemistry-Paraffin: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - Staining of paraffin-embedded Human tonsil using anti-Spermine synthase mouse monoclonal antibody.
Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900]

Flow Cytometry: Spermine synthase Antibody (OTI3C9) [NBP2-00900] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Spermine synthase antibody, and then analyzed by flow cytometry.

Applications for Spermine synthase Antibody (OTI3C9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.92 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Spermine synthase

The protein encoded by this gene belongs to the spermidine/spermine synthases family. This gene encodes an ubiquitous enzyme of polyamine metabolism. [provided by RefSeq].

Alternate Names

EC 2.5.1.22, MRSRSpS, Snyder-Robinson X-linked mental retardation syndrome, Spermidine aminopropyltransferase, spermine synthase, SPMSYSRS

Entrez Gene IDs

6611 (Human)

Gene Symbol

SMS

Additional Spermine synthase Products

Product Documents for Spermine synthase Antibody (OTI3C9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Spermine synthase Antibody (OTI3C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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