Spermine synthase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87812

Novus Biologicals, part of Bio-Techne
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NBP1-87812
NBP1-87812-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Spermine synthase Antibodies »

Product Summary for Spermine synthase Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSTLDFMLGAKADGETILKGLQSIFQEQGMAESVHTWQDHGYLATYTNKNGSFANLRIYPHGLVLLDLQSYDGDAQGKEEIDSILNKVEERMKELS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spermine synthase Antibody

Western Blot: Spermine synthase Antibody [NBP1-87812]

Western Blot: Spermine synthase Antibody [NBP1-87812]

Western Blot: Spermine synthase Antibody [NBP1-87812] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Spermine synthase Antibody [NBP1-87812]

Immunocytochemistry/ Immunofluorescence: Spermine synthase Antibody [NBP1-87812]

Immunocytochemistry/Immunofluorescence: Spermine synthase Antibody [NBP1-87812] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Spermine synthase Antibody [NBP1-87812]

Immunohistochemistry-Paraffin: Spermine synthase Antibody [NBP1-87812]

Immunohistochemistry-Paraffin: Spermine synthase Antibody [NBP1-87812] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
View 3 more images

Applications for Spermine synthase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Simple Western reported by internal validation: Tested in HeLa; separated by size; antibody dilution of 1:50.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spermine synthase

Spermine synthase is 1 of 4 enzymes involved in the synthesis of polyamines from arginine and methionine. The reaction catalysed is S-adenosylmethioninamine + spermidine = methylthioadenosine + spermine.

Alternate Names

EC 2.5.1.22, MRSRSpS, Snyder-Robinson X-linked mental retardation syndrome, Spermidine aminopropyltransferase, spermine synthase, SPMSYSRS

Gene Symbol

SMS

Additional Spermine synthase Products

Product Documents for Spermine synthase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Spermine synthase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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