SPFH2 Antibody (9T3M5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15305

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15305-100ul
NBP3-15305-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9T3M5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPFH2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SPFH2 (O94905). HLMLPFITSYKSVQTTLQTDEVKNVPCGTSGGVMIYFDRIEVVNFLVPNAVYDIVKNYTADYDKALIFNKIHHELNQFCSVHTLQEVYIELFDQIDENLKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPFH2 Antibody (9T3M5)

Western Blot: SPFH2 Antibody (9T3M5) [NBP3-15305]

Western Blot: SPFH2 Antibody (9T3M5) [NBP3-15305]

Western Blot: SPFH2 Antibody (9T3M5) [NBP3-15305] - Western blot analysis of extracts of various cell lines, using SPFH2 antibody (NBP3-15305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305]

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305]

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305] - Immunohistochemistry of paraffin-embedded rat ovary using SPFH2 Rabbit mAb (NBP3-15305) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305]

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305]

Immunohistochemistry-Paraffin: SPFH2 Antibody (9T3M5) [NBP3-15305] - Immunohistochemistry of paraffin-embedded mouse kidney using SPFH2 Rabbit mAb (NBP3-15305) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for SPFH2 Antibody (9T3M5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPFH2

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Alternate Names

C8orf2SPFH domain-containing protein 2, Endoplasmic reticulum lipid raft-associated protein 2, ER lipid raft associated 2, Erlin-2, MGC87072, NET32, SPFH domain family, member 2, SPFH2chromosome 8 open reading frame 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Gene Symbol

ERLIN2

Additional SPFH2 Products

Product Documents for SPFH2 Antibody (9T3M5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SPFH2 Antibody (9T3M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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