SPG20 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47203
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EASGTDVKQLDQGNK corresponding to internal region according to NP_055902.1.
Epitope
C-EASGTDVKQLDQGNK
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SPG20 Antibody
![Western Blot: SPG20 Antibody [NBP1-47203]](https://resources.bio-techne.com/images/products/SPG20-Antibody-Western-Blot-NBP1-47203-img0005.jpg "Western Blot: SPG20 Antibody [NBP1-47203]")
Western Blot: SPG20 Antibody [NBP1-47203]
Western Blot: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP1-47203]](https://resources.bio-techne.com/images/products/SPG20-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47203-img0004.jpg "Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP1-47203]")
Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP1-47203]
Immunocytochemistry/Immunofluorescence: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.Applications for SPG20 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
1 - 3 ug/ml
Application Notes
IF: HeLa show dimished signals after knock down of expresssion by introduction of siRNA. WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 72.8 kDa band according to NP_055902.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPG20
Alternate Names
KIAA0610SPARTIN, spastic paraplegia 20 (Troyer syndrome), Spastic paraplegia 20 protein, TAHCCP1spartin, Trans-activated by hepatitis C virus core protein 1
Entrez Gene IDs
23111 (Human)
Gene Symbol
SPART
UniProt
Additional SPG20 Products
Product Documents for SPG20 Antibody
Product Specific Notices for SPG20 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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