SPG3A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93512

Novus Biologicals, part of Bio-Techne
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NBP2-93512-0.02ml
NBP2-93512-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPG3A Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATL1 (NP_056999.2). MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPG3A Antibody - BSA Free

Western Blot: SPG3A AntibodyBSA Free [NBP2-93512]

Western Blot: SPG3A AntibodyBSA Free [NBP2-93512]

Western Blot: SPG3A Antibody [NBP2-93512] - Analysis of extracts of various cell lines, using SPG3A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: SPG3A Antibody - BSA Free [NBP2-93512]

Immunocytochemistry/ Immunofluorescence: SPG3A Antibody - BSA Free [NBP2-93512]

Immunocytochemistry/Immunofluorescence: SPG3A Antibody [NBP2-93512] - Analysis of A549 cells using ATL1 antibody. Blue: DAPI for nuclear staining

Applications for SPG3A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPG3A

SPG3A is encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene.

Long Name

Atlastin-1

Alternate Names

ATL1, GBP-3, GBP3, hGBP3

Gene Symbol

ATL1

Additional SPG3A Products

Product Documents for SPG3A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SPG3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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