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Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40449

Novus Biologicals, part of Bio-Techne
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NB110-40449

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Predicted:

Bat (93%), Canine (93%), Primate (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Synthetic 15 amino acid peptide from N-terminus of human Sphingosine Kinase 1/SPHK1.

Epitope

N-terminal domain of human

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Gorilla (93%), Equine (87%), Mouse (80%), Rat (80%), Bovine (80%).

Localization

Cytoplasmic

Specificity

Human Sphingosine Kinase 1/SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449]

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449]

Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.

Applications for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine kinase (SPHK) phosphorylates sphingosine to form a bioactive lipid mediate, sphingosine 1-phosphate (S1P or SPP). S1P is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival while extracellularly it is a ligand for EDG 1. Expression of SPHK1 and subsequent production of S1P promotes the G1-S transition, protects cells from apoptosis, stimulates migration and angiogenesis and induces tumor formation in mice.

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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