Sphingosine Kinase 1/SPHK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22974
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974]](https://resources.bio-techne.com/images/products/Sphingosine-Kinase-1-SPHK1-Antibody-Western-Blot-NBP1-22974-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
sphingosine kinase 1
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 28175299).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974]
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitopeApplications for Sphingosine Kinase 1/SPHK1 Antibody
Application
Recommended Usage
Immunoprecipitation
5 -15 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
This antibody is useful for western blot and immunoprecipitation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:28175299).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Sphingosine Kinase 1/SPHK1
Additional Sphingosine Kinase 1/SPHK1 Products
Product Documents for Sphingosine Kinase 1/SPHK1 Antibody
Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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