Sphingosine Kinase 1/SPHK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20472
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Sphingosine Kinase 1/SPHK1. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody
![Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472]](https://resources.bio-techne.com/images/products/Sphingosine-Kinase-1-SPHK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20472-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472]")
Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472]
Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] -
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Sphingosine Kinase 1/SPHK1 antibody (NBP2-20472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Sphingosine Kinase 1/SPHK1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Sphingosine Kinase 1/SPHK1
Additional Sphingosine Kinase 1/SPHK1 Products
Product Documents for Sphingosine Kinase 1/SPHK1 Antibody
Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.