Sphingosine Kinase 2/SPHK2 Antibody (M532)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23422
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M532
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Sphingosine Kinase 2/SPHK2 Antibody (M532)
Immunogen
Clone M532 was generated from a Sphingosine Kinase 2/SPHK2 (N-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human Sphingosine Kinase 2/SPHK2. This peptide sequence is highly conserved in rat and mouse Sphingosine Kinase 2/SPHK2 proteins, and has no homology to SK1. [UniProt# Q9NRA0]
Specificity
The antibody detects 70 kDa proteins corresponding to the molecular mass of Sphingosine Kinase 2/SPHK2 on SDS-PAGE immunoblots of human recombinant Sphingosine Kinase 2/SPHK2 and endogenous Sphingosine Kinase 2/SPHK2 in human HeLa cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Sphingosine Kinase 2/SPHK2 Antibody (M532)
Western Blot: Sphingosine Kinase 2/SPHK2 Antibody (M532) [NBP3-23422] -
Western Blot: Sphingosine Kinase 2/SPHK2 Antibody (M532) [NBP3-23422] - Western blot of human recombinant Sphingosine Kinase 2/SPHK2 (lanes 1-4). The blots were probed with rabbit polyclonal anti-Sphingosine Kinase 2/SPHK2 (N-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Sphingosine Kinase 2/SPHK2 (N-terminal region) at 1:250 (lane 3) or 1:1000 (lane 4).Applications for Sphingosine Kinase 2/SPHK2 Antibody (M532)
Application
Recommended Usage
Western Blot
1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Sphingosine Kinase 2/SPHK2
Alternate Names
SK2, SPK2
Gene Symbol
SPHK2
Additional Sphingosine Kinase 2/SPHK2 Products
Product Documents for Sphingosine Kinase 2/SPHK2 Antibody (M532)
Product Specific Notices for Sphingosine Kinase 2/SPHK2 Antibody (M532)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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