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Splicing Factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32583

Novus Biologicals, part of Bio-Techne
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NBP1-32583

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (99%), Rabbit (100%), Rat (99%), Xenopus (96%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Splicing Factor 1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Splicing Factor 1 Antibody

Western Blot: Splicing Factor 1 Antibody [NBP1-32583]

Western Blot: Splicing Factor 1 Antibody [NBP1-32583]

Western Blot: Splicing Factor 1 Antibody [NBP1-32583] - Sample(30 ug whole cell lysate)A:Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-32583]

Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-32583]

Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody [NBP1-32583] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.

Applications for Splicing Factor 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Splicing Factor 1

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells.

Alternate Names

Mammalian branch point-binding protein, mBBP, splicing factor 1, Transcription factor ZFM1, ZFM1BBP, Zinc finger gene in MEN1 locus, Zinc finger protein 162D11S636, ZNF162MBBP

Gene Symbol

SF1

Additional Splicing Factor 1 Products

Product Documents for Splicing Factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Splicing Factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.