SPOP Antibody (JE36-04)

Western Blot: SPOP Antibody (JE36-04) [NBP3-32976]

Western Blot: SPOP Antibody (JE36-04) [NBP3-32976] - Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (NBP3-32976) at 1/500 dilution.

Lane 1: K562 cell lysate
Lane 2: LOVO cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32976) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.