SPOP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33090

Novus Biologicals, part of Bio-Techne
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NBP1-33090
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPOP Antibodies »

Product Summary for SPOP Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPOP. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPOP Antibody

Western Blot: SPOP Antibody [NBP1-33090]

Western Blot: SPOP Antibody [NBP1-33090]

Western Blot: SPOP Antibody [NBP1-33090] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: SPOP Antibody [NBP1-33090]

Western Blot: SPOP Antibody [NBP1-33090]

Western Blot: SPOP Antibody [NBP1-33090] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:2000.
SPOP Antibody

Western Blot: SPOP Antibody [NBP1-33090] -

Western Blot: SPOP Antibody [NBP1-33090] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SPOP antibody [N1C1] (NBP1-33090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SPOP Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
Immunohistochemistry (IHC) Assay-dependent dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPOP

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

HIB homolog 1, Roadkill homolog 1, speckle-type POZ protein, TEF2

Gene Symbol

SPOP

Additional SPOP Products

Product Documents for SPOP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPOP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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