SPRED1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57368

Novus Biologicals, part of Bio-Techne
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NBP2-57368
NBP2-57368-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPRED1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPRED1 Antibody

Western Blot: SPRED1 Antibody [NBP2-57368]

Western Blot: SPRED1 Antibody [NBP2-57368]

Western Blot: SPRED1 Antibody [NBP2-57368] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.
Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody [NBP2-57368]

Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody [NBP2-57368]

Immunocytochemistry/Immunofluorescence: SPRED1 Antibody [NBP2-57368] - Staining of human cell line U-2 OS shows localization to nucleus.

Applications for SPRED1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRED1

SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N-terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6 - 123), a central KBD domain that binds to the SCFR (aa 233 - 285), and a C-terminal Cys-rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334 - 442). There is one splice variant that shows a nine Lys substitution for aa 269 - 444.

Long Name

Sprouty-related with an EVH1 Domain Containing-1

Alternate Names

NFLS

Gene Symbol

SPRED1

Additional SPRED1 Products

Product Documents for SPRED1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SPRED1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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