SPRED1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81927

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SPRED1 Antibodies »

Product Specifications

Immunogen

SPRED1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human SPRED1. The immunogen is located within amino acids 250 - 300 of SPRED1. Amino Acid Squence: RHPDMWKNDLERDD

Specificity

This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

498 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPRED1 Antibody - BSA Free

Western Blot: SPRED1 AntibodyBSA Free [NBP2-81927]

Western Blot: SPRED1 AntibodyBSA Free [NBP2-81927]

Western Blot: SPRED1 Antibody [NBP2-81927] - Analysis of SPRED1 in human brain tissue lysate with SPRED1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody - BSA Free [NBP2-81927]

Immunocytochemistry/ Immunofluorescence: SPRED1 Antibody - BSA Free [NBP2-81927]

Immunocytochemistry/Immunofluorescence: SPRED1 Antibody [NBP2-81927] - Analysis of SPRED1 in human brain tissue with SPRED1 antibody at 20 ug/ml.
Immunohistochemistry: SPRED1 Antibody - BSA Free [NBP2-81927]

Immunohistochemistry: SPRED1 Antibody - BSA Free [NBP2-81927]

Immunohistochemistry: SPRED1 Antibody [NBP2-81927] - Analysis of SPRED1 in human brain tissue with SPRED1 antibody at 2.5 ug/ml.
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Applications for SPRED1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRED1

SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N-terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6 - 123), a central KBD domain that binds to the SCFR (aa 233 - 285), and a C-terminal Cys-rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334 - 442). There is one splice variant that shows a nine Lys substitution for aa 269 - 444.

Long Name

Sprouty-related with an EVH1 Domain Containing-1

Alternate Names

NFLS

Gene Symbol

SPRED1

Additional SPRED1 Products

Product Documents for SPRED1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPRED1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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