SPRR3 Antibody (4A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006707-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 4A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPRR3 Antibodies »

Product Specifications

Immunogen

SPRR3 (AAH17802, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKVPEPCPSTVTPGPAQQKTKQK

Specificity

SPRR3 - small proline-rich protein 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SPRR3 Antibody (4A12)

Western Blot: SPRR3 Antibody (4A12) [H00006707-M01]

Western Blot: SPRR3 Antibody (4A12) [H00006707-M01]

Western Blot: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of SPRR3 expression in transfected 293T cell line by SPRR3 monoclonal antibody (M01), clone 4A12.Lane 1: SPRR3 transfected lysate(18.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: SPRR3 Antibody (4A12) [H00006707-M01]

Immunohistochemistry-Paraffin: SPRR3 Antibody (4A12) [H00006707-M01]

Immunohistochemistry-Paraffin: SPRR3 Antibody (4A12) [H00006707-M01] - Analysis of monoclonal antibody to SPRR3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: SPRR3 Antibody (4A12) [H00006707-M01] - Detection limit for recombinant GST tagged SPRR3 is approximately 0.03ng/ml as a capture antibody.

Applications for SPRR3 Antibody (4A12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRR3

SPRR3, also known as Small proline-rich protein 3, consists of a 169 amino acid isoform that is 18 kDa, and is involved in the surrounding and protection of keratinocytes. Disease research on the protein is currently being conducted with ichthyosis vulgaris, psoriasis, eosinophilic esophagitis, verrucous carcinoma, periodontitis, esophageal cancer, eczema, tonsillitis, and skin disease. SPRR3 is linked to several biological processes, including epidermis development, wound healing, and keratinocyte differentiation, in which it interacts with other proteins, such as IVL, EVPL, NFKBIA, and LOR.

Alternate Names

22 kDa pancornulin, Cornifin beta, Esophagin, small proline-rich protein 3, SPRC

Entrez Gene IDs

6707 (Human)

Gene Symbol

SPRR3

OMIM

182271 (Human)

UniProt

AAH17802 (Human)

Additional SPRR3 Products

Product Documents for SPRR3 Antibody (4A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPRR3 Antibody (4A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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