SPRY1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51918
![Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918]](https://resources.bio-techne.com/images/products/SPRY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51918-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CPSRGQGKPS corresponding to C-Terminus according to NP_005832.1, NP_955359.1.
Epitope
CPSRGQGKPS
Specificity
NP_005832.1 and NP_955359.1 represent varients of the same protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
NP_005832.1 and NP_955359.1 represent varients of the same protein.
Scientific Data Images for SPRY1 Antibody
![Western Blot: SPRY1 Antibody [NBP1-51918]](https://resources.bio-techne.com/images/products/SPRY1-Antibody-Western-Blot-NBP1-51918-img0001.jpg "Western Blot: SPRY1 Antibody [NBP1-51918]")
Western Blot: SPRY1 Antibody [NBP1-51918]
Western Blot: SPRY1 Antibody [NBP1-51918] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918]](https://resources.bio-techne.com/images/products/SPRY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51918-img0004.jpg "Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918]")
Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918]
Immunocytochemistry/Immunofluorescence: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Golgi apparatus staining. The nucl![Flow Cytometry: SPRY1 Antibody [NBP1-51918]](https://resources.bio-techne.com/images/products/SPRY1-Antibody-Flow-Cytometry-NBP1-51918-img0003.jpg "Flow Cytometry: SPRY1 Antibody [NBP1-51918]")
Flow Cytometry: SPRY1 Antibody [NBP1-51918]
Flow Cytometry: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line)Applications for SPRY1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
1-3 ug/ml
Immunohistochemistry-Paraffin
1 - 3 ug/ml
Peptide ELISA
detection limit 1:16000
Western Blot
0.3-1 ug/ml
Application Notes
WB successful usage in ERMS cells is reported in scientific literature (PMID: 20068162).
Western blot: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162).
IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells.
Flow Cytometry: Flow cytometric analysis of HEK293 cells.
Western blot: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162).
IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells.
Flow Cytometry: Flow cytometric analysis of HEK293 cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPRY1
Long Name
Sprouty Homolog 1
Alternate Names
Drosophila, homolog of, 1 (antagonist of FGF signaling), sprouty homolog 1, antagonist of FGF signaling (Drosophila), spry-1
Entrez Gene IDs
10252 (Human)
Gene Symbol
SPRY1
UniProt
Additional SPRY1 Products
Product Documents for SPRY1 Antibody
Product Specific Notices for SPRY1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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