SPRY2 Antibody (10M8H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33538
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 10M8H1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 216-315 of human SPRY2 (O43597).
Sequence:
GTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
Sequence:
GTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SPRY2 Antibody (10M8H1)
Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] -
Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Human appendix using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Western Blot: SPRY2 Antibody (10M8H1) [NBP3-33538] -
Western Blot: SPRY2 Antibody (10M8H1) [NBP3-33538] - Western blot analysis of lysates from C6 cells, using SPRY2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] -
Immunohistochemistry: SPRY2 Antibody (10M8H1) [NBP3-33538] - Immunohistochemistry analysis of paraffin-embedded Rat pancreas using SPRY2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.Applications for SPRY2 Antibody (10M8H1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPRY2
Long Name
Sprouty Homolog 2
Alternate Names
sprouty 2
Gene Symbol
SPRY2
Additional SPRY2 Products
Product Documents for SPRY2 Antibody (10M8H1)
Product Specific Notices for SPRY2 Antibody (10M8H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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