SPRY2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13158
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SPRY2 Antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with peptide corresponding to an internal region of human sprouty-2. (Uniprot: O43597)
Reactivity Notes
A BLAST analysis was used to suggest reactivity with SPRY2 from human, monkey, and orangutan based on a 100% homology with the immunizing sequence. Cross-reactivity with Sprouty2 from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for SPRY2 Antibody
Immunohistochemistry: SPRY2 Antibody [NBP2-13158]
Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: liver. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.Immunohistochemistry-Paraffin: SPRY2 Antibody [NBP2-13158]
Immunohistochemistry-Paraffin: SPRY2 Antibody [NBP2-13158] - Immunohistochemistry-Paraffin: Sprouty 2 - Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.Immunohistochemistry: SPRY2 Antibody [NBP2-13158]
Immunohistochemistry: SPRY2 Antibody [NBP2-13158] - Tissue: kidney. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-sprouty 2 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for SPRY2 Antibody
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1:500-1:5000
Application Notes
This product has been tested by ELISA, Immunofluorescence, Immunohistochemistry, and is suitable for western blotting. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SPRY2
Additional SPRY2 Products
Product Documents for SPRY2 Antibody
Product Specific Notices for SPRY2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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