SPRY4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20479

Novus Biologicals, part of Bio-Techne
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NBP2-20479
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Rat (94%), Sheep (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPRY4 Antibodies »

Product Summary for SPRY4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPRY4. The exact sequence is proprietary.

Localization

Cytoplasm; Cell projection; ruffle membrane; Peripheral membrane protein; Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPRY4 Antibody

Western Blot: SPRY4 Antibody [NBP2-20479]

Western Blot: SPRY4 Antibody [NBP2-20479]

Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]

Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]

Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded mouse duodenum. SPRY4 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]

Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479]

Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded U87 xenograft. SPRY4 antibody dilution: 1:500.
View 1 more images

Applications for SPRY4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRY4

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS (see HRAS; MIM 190020) activation and impairs the formation of active GTP-RAS (Leeksma et al., 2002 [PubMed 12027893]).[supplied by OMIM]

Long Name

Sprouty Homolog 4

Alternate Names

protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry-4

Gene Symbol

SPRY4

UniProt

Q9C004 (Human)

Additional SPRY4 Products

Product Documents for SPRY4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPRY4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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