Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SPT3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SUPT3H (NP_852001) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPT3 Antibody (OTI1A5) - Azide and BSA Free

Western Blot: SPT3 Antibody (OTI1A5)Azide and BSA Free [NBP2-74348]

Western Blot: SPT3 Antibody (OTI1A5)Azide and BSA Free [NBP2-74348]

Western Blot: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPT3.
Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348]

Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348]

Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348]

Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348]

Immunohistochemistry: SPT3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-74348] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for SPT3 Antibody (OTI1A5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPT3

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.

Alternate Names

SPT3-like protein, SPT3SPT3L, suppressor of Ty (S.cerevisiae) 3 homolog, suppressor of Ty 3 homolog (S. cerevisiae), transcription initiation protein SPT3 homolog

Gene Symbol

SUPT3H

Additional SPT3 Products

Product Documents for SPT3 Antibody (OTI1A5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPT3 Antibody (OTI1A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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