Spt6 Antibody

Western Blot: Spt6 Antibody [NB100-2582] -

Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Induces lncRNA Transcription(A) YWHAZ locus showing pre-mRNA (– strand) & PROMPT (+ strand). SPT6 depletion induced lncRNA as shown by ChRNA-seq & mNET-seq analyses. The profile of mNuc-seq/H3K36me3 is shown below.(B) Meta-analysis of strand-specific ChrRNA-seq signals from −3 kb of TSS to +3 kb of transcription end site (TES) for divergent (pre-mRNA-PROMPT) genes.(C) Boxplots of PROMPT mNET-seq/total CTD & T4P signals.(D) Enhancer located 90 kb downstream of NR4A1 gene with neighboring gene ATG101 showing SPT6 depletion-induced eRNA by ChRNA-seq & mNET-seq. mNuc-seq/H3K4me1 & H3K4me3 signals indicate active enhancer & promoter, respectively.(E) Meta-analysis of eRNA from ChrRNA-seq & mNET-seq (Total) −3 kb to +3 kb from TSS (enhancers with highest eRNA levels selected).(F) Boxplots of eRNA mNET-seq/total CTD & T4P signals at TSS (–/+2 kb).(G & H) Western blot (G) & quantitative RT-PCR (H) of chromatin-bound RNA of parental & SETD2 CRISPR KO U2OS cells with indicated siRNA transfection for 48 hr. Data are represented as mean ± SEM.See also Figure S3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Spt6 Antibody [NB100-2582] -

Western Blot: Spt6 Antibody [NB100-2582] - SPT6 Depletion Causes H3K36me3 Redistribution(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 & H3 profiles shown as loading controls.(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA & enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red & blue, respectively.(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), & eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), & eRNA (2 kb from center) & across lincRNA gene bodies.(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.See also Figure S2. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30449723), licensed under a CC-BY license. Not internally tested by Novus Biologicals.