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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1553 and the C-terminus (residue 1583) of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spt6 Antibody

Immunoprecipitation: Spt6 Antibody [NB100-2584]

Immunoprecipitation: Spt6 Antibody [NB100-2584]

Immunoprecipitation: Spt6 Antibody [NB100-2584] - Detection of Human SUPT6H by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUPT6H antibodies NB100-2583, NB100-2584 and another SUPT6H Ab used for IP at 3 mcg/mg lysate (A and B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). * - The asterisk marks a protein with which BL3468 interacts in IP and WB. This band is likely to be a cross-reacting protein.

Applications for Spt6 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
NB100-2582 or NB100-2583 are recommended for Western Blot.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Spt6

Spt6 is a histone H3-H4 chaperone protein that mediates chromatin reassembly behind RNA polymerase II. Spt6 binds both histone H3 and H4 and deposits them on to DNA, which leads to transcriptional repression of target genes.

Alternate Names

emb-5, hSPT6, KIAA0162MGC87943, SPT6HSPT6, suppressor of Ty (S.cerevisiae) 6 homolog, suppressor of Ty 6 homolog (S. cerevisiae), Tat-cotransactivator 2 protein, Tat-CT2 protein, transcription elongation factor SPT6

Entrez Gene IDs

6830 (Human)

Gene Symbol

SUPT6H

UniProt

Additional Spt6 Products

Product Documents for Spt6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Spt6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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