SPTLC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59643

Novus Biologicals, part of Bio-Techne
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NBP1-59643
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SPTLC1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to SPTLC1(serine palmitoyltransferase, long chain base subunit 1) The peptide sequence was selected from the middle region of SPTLC1. Peptide sequence ICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLI. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SPTLC1 Antibody

Western Blot: SPTLC1 Antibody [NBP1-59643]

Western Blot: SPTLC1 Antibody [NBP1-59643]

Western Blot: SPTLC1 Antibody [NBP1-59643] - HepG2 cell lysate, concentration 0.5ug/ml.
Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-59643]

Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-59643]

Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-59643] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: SPTLC1 Antibody [NBP1-59643]

Western Blot: SPTLC1 Antibody [NBP1-59643]

Western Blot: SPTLC1 Antibody [NBP1-59643] - SPTLC1 levels in human neuroblastoma cells. Cells were untreated or treated with various drugs. Image from verified customer review.

Applications for SPTLC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPTLC1

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1.Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Long Name

Serine palmitoyltransferase 1

Alternate Names

LCB 1, LCB1, SPT 1, SPT1

Gene Symbol

SPTLC1

UniProt

O15269 (Human)

Additional SPTLC1 Products

Product Documents for SPTLC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPTLC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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