SR-AI/MSR Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00092

Novus Biologicals, part of Bio-Techne
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NBP1-00092
NBP1-00092SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (93%), Canine (100%), Equine (100%), Primate (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.00 mg/ml

View all SR-AI/MSR Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human Macrophage Scavenger Receptor I (within residues 400-450).

Localization

Membrane; Single-pass type II membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SR-AI/MSR Antibody - BSA Free

Western Blot: SR-AI/MSR AntibodyBSA Free [NBP1-00092]

Western Blot: SR-AI/MSR AntibodyBSA Free [NBP1-00092]

Western Blot: SR-AI/MSR Antibody [NBP1-00092] - Detection of Macrophage Scavenger Receptor I (MSR1) protein in the lysate of human liver.
Immunocytochemistry/ Immunofluorescence: SR-AI/MSR Antibody - BSA Free [NBP1-00092]

Immunocytochemistry/ Immunofluorescence: SR-AI/MSR Antibody - BSA Free [NBP1-00092]

Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00092] - The MSR antibody was tested in Raw cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Frozen: SR-AI/MSR Antibody - BSA Free [NBP1-00092]

Immunohistochemistry-Frozen: SR-AI/MSR Antibody - BSA Free [NBP1-00092]

Immunohistochemistry-Frozen: SR-AI/MSR Antibody - BSA Free [NBP1-00092] - Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs and C-type lectins. NPCs from the 25-45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, and double immune-labeled with antibodies to Fc-gamma-RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), and to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green and red fluorescence is seen as yellow staining in the overlay images. Cell nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.
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Applications for SR-AI/MSR Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5 ug/million cells

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

reported in scientific literature (PMID 26358193)

Proximity Ligation Assay

reported in scientific literature (PMID 28338748)

Western Blot

0.5 ug/ml
Application Notes
In Western blot, a band is seen ~50 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-00092 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.00 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SR-AI/MSR

Macrophage Scavenger Receptor I (MSR1, also called CD204 or scavenger receptor A) belongs to scavenger receptor A family and represents phagocytic pattern-recognition receptors (PRRs) expressed predominantly on macrophages and dendritic cells. MSR1 is a single-pass type II membrane glycoprotein capable of binding to a wide range of polyanionic ligands (e.g., modified low density lipoproteins, pathogen-derived molecules), and is implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis, innate immune response as well as Alzheimer's disease. MSR1 mediates the endocytosis of a diverse group of macromolecules including modified LDLs, and scavenger receptors MSR1, CD36 and LOX-1 are three main receptors for oxidized LDL on macrophage membrane wherein MSR1/CD36 account for more than 9/10 of the lipid accumulation in macrophages exposed to oxidized LDL. Defects in MSR1 have also been linked to prostate cancer and Barrett esophagus (BE).

Long Name

Macrophage Scavenger Receptor Types I and II

Alternate Names

CD204, MSR1, SCARA1, SRAI

Gene Symbol

MSR1

Additional SR-AI/MSR Products

Product Documents for SR-AI/MSR Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SR-AI/MSR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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