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SREC-I/SCARF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1456

Novus Biologicals, part of Bio-Techne
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NB100-1456

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence SELDPKGQHVC corresponding to internal region according to NP_003684.2, NP_663325.1, NP_663327.2.

Epitope

Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 protein.

Localization

Type I membrane protein

Specificity

This antibody is expected to recognise all three reported isoforms (NP_003684.2; NP_663325.1; NP_663327.2). Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SREC-I/SCARF1 Antibody

Western Blot: SREC-I/SCARF1 Antibody [NB100-1456]

Western Blot: SREC-I/SCARF1 Antibody [NB100-1456]

Western Blot: SREC-I/SCARF1 Antibody [NB100-1456] - Human Heart lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Antibody at 0.3 ug/mL, 1 hr incubation. Detected by chemiluminescence.

Applications for SREC-I/SCARF1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1.0 ug/mL
Application Notes
WB: Approx. 85-90 kDa band observed in human heart lysates (calculated MW of 87.4 kDa band according to NP_003684). An additional faint band of 28 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SREC-I/SCARF1

The protein recognised by this antibody is a scavenger receptor that is expressed in endothelial cells and mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Transcription factors ZNF444/EZF-2 and SP1 are found to be responsible for the regulation of this gene. Five alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Scavenger Receptor Expressed by Endothelial Cells 1

Alternate Names

SCARF1, SR-F1, SRECI

Entrez Gene IDs

8578 (Human)

Gene Symbol

SCARF1

Additional SREC-I/SCARF1 Products

Product Documents for SREC-I/SCARF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SREC-I/SCARF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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