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SRI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55404

Novus Biologicals, part of Bio-Techne
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NBP2-55404

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRI Antibody

Western Blot: SRI Antibody [NBP2-55404]

Western Blot: SRI Antibody [NBP2-55404]

Western Blot: SR1 Antibody [NBP2-55404] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: SRI Antibody [NBP2-55404]

Immunocytochemistry/ Immunofluorescence: SRI Antibody [NBP2-55404]

Immunocytochemistry/Immunofluorescence: SR1 Antibody [NBP2-55404] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SRI Antibody [NBP2-55404]

Immunohistochemistry-Paraffin: SRI Antibody [NBP2-55404]

Immunohistochemistry-Paraffin: SR1 Antibody [NBP2-55404] - Staining of human cerebral cortex.

Applications for SRI Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRI

Sorcin (SRI) is a Ca2+-binding protein first identified in multidrug-resistant cells. This protein is widely distributed in mammalian tissues, including heart and skeletal muscle. At the subcellular level, sorcin localizes to T-tubule junctions of cardiac SR and co-localizes with brain ryanodine receptors in rat brain caudate-putamen nucleus.

Alternate Names

calcium binding protein amplified in mutlidrug-resistant cells, CP22, CP-22, FLJ26259,22 kDa protein, H_RG167B05.1, SCN, sorcin, V19

Gene Symbol

SRI

Additional SRI Products

Product Documents for SRI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SRI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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