SRP54 Antibody (1O3J1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33390
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 1O3J1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-504 of human SRP54 (P61011).
Sequence:
PGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM
Sequence:
PGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SRP54 Antibody (1O3J1)
Immunocytochemistry/ Immunofluorescence: SRP54 Antibody (1O3J1) [NBP3-33390] -
Immunocytochemistry/ Immunofluorescence: SRP54 Antibody (1O3J1) [NBP3-33390] - Immunofluorescence analysis of C6 cells using SRP54 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SRP54 Antibody (1O3J1) [NBP3-33390] -
Immunocytochemistry/ Immunofluorescence: SRP54 Antibody (1O3J1) [NBP3-33390] - Immunofluorescence analysis of NIH-3T3 cells using SRP54 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: SRP54 Antibody (1O3J1) [NBP3-33390] -
Western Blot: SRP54 Antibody (1O3J1) [NBP3-33390] - Western blot analysis of various lysates using SRP54 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for SRP54 Antibody (1O3J1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SRP54
Alternate Names
signal recognition particle 54 kDa protein, signal recognition particle 54kD, signal recognition particle 54kDa
Gene Symbol
SRP54
Additional SRP54 Products
Product Documents for SRP54 Antibody (1O3J1)
Product Specific Notices for SRP54 Antibody (1O3J1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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