SRPK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30108

Novus Biologicals, part of Bio-Techne
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NBP1-30108
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SRPK1 Antibodies »

Product Summary for SRPK1 Antibody

Immunogen

maps to a region between residue 275 and 325 of human SFRS protein kinase 1 using the numbering given in entry NP_003128.3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRPK1 Antibody

Western Blot: SRPK1 Antibody [NBP1-30108]

Western Blot: SRPK1 Antibody [NBP1-30108]

Western Blot: SRPK1 Antibody [NBP1-30108] - Detection of human and mouse SRPK1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRPK1 antibody NBP1-30108 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SRPK1 was also immunoprecipitated by another rabbit anti-SRPK1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108]

Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108]

Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-30108] - Section of human testicular seminoma. Antibody: Affinity purified rabbit anti- SRPK1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for SRPK1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 -1:500

Immunohistochemistry-Paraffin

1:100 -1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SRPK1

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.

Long Name

SRSF protein kinase 1

Alternate Names

EC 2.7.11.1, Serine/arginine-rich protein-specific kinase 1, serine/arginine-rich splicing factor kinase 1, serine/threonine-protein kinase SRPK1, SFRS protein kinase 1SFRSK1, SR protein kinase 1, SR-protein-specific kinase 1, SRSF protein kinase 1

Gene Symbol

SRPK1

Additional SRPK1 Products

Product Documents for SRPK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SRPK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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